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Becton Dickinson
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Image Search Results
Journal: Drug Metabolism and Disposition
Article Title: Characterization of Differential Cocaine Metabolism in Mouse and Rat through Metabolomics-Guided Metabolite Profiling
doi: 10.1124/dmd.112.048678
Figure Lengend Snippet: In vitro metabolism of cocaine by pooled human, mouse, and rat liver microsomes. (A–C) Representative chromatogram of major phase I cocaine metabolites formed by MLM, RLM, and HLM, respectively. Ions within the 20 ppm range of theoretical accurate mass ([M+H]+) of cocaine (I) and its metabolites (200.1287, 290.1392, 306.1341, 320.1498, and 336.1447 m/z) were extracted and labeled in the chromatograms. The ion intensity of the most abundant metabolites was set arbitrarily as 100%. Major phase I metabolites are labeled. The chemical identities of these metabolites are presented in the Table 1. One unknown metabolite is labeled as “UK”. (D) A comparison of phase I cocaine metabolites formed by microsomal incubations (n = 3). Relative abundance of individual cocaine metabolite formed by MLM, RLM, and HLM was represented as a relative peak area (area % ± S.D.) by calculating its percentage in the total peak area of all detected phase I cocaine metabolites.
Article Snippet: Pooled
Techniques: In Vitro, Labeling, Comparison